FastQCFastQC Report
Thu 2 Jun 2016
DRR028327_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028327_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414696
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42180.2981559289062809No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11700.048.935161
TCAACGC16700.045.557534
ATCAACG17150.044.784643
GTATCAA18150.044.7219961
CAACGCA18800.040.858425
AACGCAG19500.040.506576
GTACATG34300.038.455381
ACGCAGA21250.035.806677
TATCAAC22400.034.6190832
CGCAGAG22250.032.8969578
TACATGG36700.031.9910722
ACATGGG40750.026.4939083
CCTATAC6100.026.1324543
GAGTACA25850.022.989691
CATGGGG35700.022.3260464
TCTGTCG3655.7152647E-921.8405828
GCAGAGT29650.021.2661959
CGCTCTA2155.0711946E-420.224472145
TATACGC1850.005064756619.5832884
TAGTACC5255.0931703E-1119.3221784
Produced by FastQC (version 0.11.5)