FastQCFastQC Report
Thu 2 Jun 2016
DRR028327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414696
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.22563151376691531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.19057097779310891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15280.10800907049995194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20100.071.096131
GTATCAA37850.062.6697121
ATCAACG43300.055.2354553
TCAACGC43350.055.1717454
CAACGCA44800.053.256285
AACGCAG46700.051.5553936
TATCAAC48600.048.4661982
ACGCAGA53300.045.0353667
CGCAGAG54550.044.0033958
GCAGAGT64850.036.678949
GTACATG37400.031.0330311
TACATGG38850.028.5425192
CTAGACG1306.497056E-427.8752354
ACATGGG38800.027.4585443
GTGGTAT11300.026.961661
CAGAGTA62400.024.5217139
CATGGGG33050.023.4641174
TGGTATC11500.023.3182412
GTACCGT1600.002172249422.6622456
TGTACCG2603.1983673E-622.3135955
Produced by FastQC (version 0.11.5)