FastQCFastQC Report
Thu 2 Jun 2016
DRR028326_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028326_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722463
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49690.2884822489655801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17800.049.2563934
ATCAACG18250.048.437483
GGTATCA14000.046.073141
GTATCAA21750.043.984591
CAACGCA20600.043.2673655
AACGCAG21450.041.8894166
GTACATG40900.038.0978971
ACGCAGA22750.037.9053767
TATCAAC25150.035.442772
TACATGG43000.035.392512
CGCAGAG23600.034.6987888
ACATGGG46300.030.5167473
CATGGGG40600.025.343094
GCAGAGT34550.021.8145289
GAGTACA29050.018.9606531
ATACGCC2307.9932937E-418.9031126
ATGGGGG33150.018.5805245
GTATATA15750.018.4062481
CAGTGCG3602.6291436E-618.1175949
TATACTG14800.017.6263895
Produced by FastQC (version 0.11.5)