FastQCFastQC Report
Thu 2 Jun 2016
DRR028326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722463
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35640.20691300771047044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.17358863441478858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.081.03381
GTATCAA45700.064.751781
ATCAACG53500.054.7277263
TCAACGC53700.054.5239034
CAACGCA55250.052.091175
TATCAAC57800.050.4055142
AACGCAG58550.049.0313876
CGCAGAG65250.044.1078538
ACGCAGA65550.043.6847957
GCAGAGT79600.035.245519
GTACATG38700.029.2362291
TACATGG39100.027.2471072
TGGTATC13000.026.2019942
GTGGTAT14050.024.7783641
CATGGGG38000.023.077124
CAGAGTA76700.023.062189
ACATGGG44000.023.0597823
CTGTGCG2603.208448E-622.3060469
TCTGTCG4358.367351E-1121.6650668
TGTACCG2307.9690886E-418.9116465
Produced by FastQC (version 0.11.5)