FastQCFastQC Report
Thu 2 Jun 2016
DRR028325_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028325_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707945
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49410.2892950299921836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG32200.043.2193341
TCAACGC14200.042.3490644
ATCAACG14650.039.5634083
TACATGG34700.035.5038642
CAACGCA16900.034.301135
GGTATCA13200.034.0447771
GTATCAA19950.033.78881
AACGCAG17250.032.7650346
ACGCAGA18600.029.2199137
CGCAGAG18550.028.5190548
TATCAAC23150.028.486992
ACATGGG41950.027.4603793
CATGGGG35900.025.0254174
CATACCG1750.003671159720.703185
CTAATAC13800.018.9001273
ATAGTAC10000.018.8371283
GAGTACA23950.017.8557951
ATGGGGG33850.017.3392965
CTACGCA3052.7569185E-416.628474
GTATATA21500.016.1821231
Produced by FastQC (version 0.11.5)