FastQCFastQC Report
Thu 2 Jun 2016
DRR028325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707945
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36970.21645896091501776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32230.18870631080040634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.10064726908653382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.086.965581
GTATCAA46800.059.8126751
TCAACGC54650.050.7917634
ATCAACG55150.050.5941053
CAACGCA57850.047.62035
AACGCAG59100.046.7357646
TATCAAC60800.046.011722
ACGCAGA65600.041.883917
CGCAGAG66150.041.3164868
GCAGAGT80350.033.0222789
GTACATG33450.031.6525361
TACATGG36500.028.9897652
ACATGGG36100.027.70493
GTGGTAT13550.024.08381
CATGGGG31850.023.6651134
CAGAGTA78500.023.1801329
CGTACAC1902.1993023E-422.886663
TATACCG1600.002176537622.6548865
TGGTATC12800.021.515842
TAATCCG1800.004309820.1376765
Produced by FastQC (version 0.11.5)