FastQCFastQC Report
Thu 2 Jun 2016
DRR028324_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028324_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220518
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.052.569361
TCAACGC20150.046.4028664
GTATCAA21950.046.2299771
ATCAACG20600.045.389213
CAACGCA24050.038.8780755
AACGCAG26450.036.172496
GTACATG44700.034.700551
TATCAAC28300.033.551742
TACATGG45700.033.3068242
ACGCAGA29100.032.1311957
CGCAGAG29000.031.9920528
ACATGGG50800.028.9642623
GAGTACA36450.026.8451841
TACGCGC1358.095474E-426.8451846
GCAGAGT36200.025.4287669
CATGGGG32250.024.722544
AGTACAT36950.023.5394862
AGAGTAC31900.022.039985
CATGGGA19100.022.010244
CAGAGTA33600.021.5720234
Produced by FastQC (version 0.11.5)