FastQCFastQC Report
Thu 2 Jun 2016
DRR028324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220518
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.10831466639574346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.10667601788748712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.067.057051
GTATCAA31250.059.0200161
ATCAACG35600.049.6890873
TCAACGC35900.049.47584
CAACGCA37850.047.1183855
AACGCAG40800.043.7115446
TATCAAC42150.042.6555252
CGCAGAG46750.037.8381088
ACGCAGA47050.037.750937
GTACATG48550.034.997921
TACATGG48900.034.3953972
GAGTACA37350.031.3004721
GCAGAGT56050.031.042519
ACATGGG52050.030.6424263
GTGGTAT10000.029.7714141
GGTTACG1050.007650627327.6621741
AGTACAT39200.027.1864472
TGGTATC12100.025.1643162
CATGGGG35900.025.0408134
ATACCGT2351.3312892E-624.6798956
Produced by FastQC (version 0.11.5)