FastQCFastQC Report
Thu 2 Jun 2016
DRR028323_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028323_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867708
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG350.003568934562.131475
TCAACGC13450.056.0492134
GTATCAA14250.055.445981
ATCAACG13750.054.8263173
GGTATCA9450.052.1597561
CAACGCA15450.049.2628145
AACGCAG16750.045.8721926
TATCAAC17500.043.492032
ACGCAGA18150.041.535097
CGCAGAG18700.040.313478
GAGCGAC2201.6370905E-1136.24336145
GTACATG27100.033.7023121
TACATGG27550.031.8362712
GCAGAGT24100.030.0774789
ACATGGG29700.029.0434993
GTATAGG3052.3646862E-1128.5193651
TACCTGG9050.024.8297062
CATGGGG22400.022.6520984
GTACCTG10900.022.6105351
GTCTACG2252.6547761E-522.5514221
Produced by FastQC (version 0.11.5)