FastQCFastQC Report
Thu 2 Jun 2016
DRR028323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867708
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.1437119399613695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11570.1333397871173252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.068.931831
GTATCAA24750.060.16941
TCAACGC27600.053.8474354
ATCAACG28000.053.0781863
TTTCGCG701.6962089E-551.7835929
CAACGCA29200.050.896895
AACGCAG30950.048.2532776
TATCAAC32000.046.6699642
ACGCAGA35100.042.341577
CGCAGAG36550.040.4634638
GCAGAGT41250.034.9743279
GTACATG26200.034.9354441
TACATGG25850.034.2152332
TATAACG1652.3867087E-630.7563172
ACATGGG27350.030.4832133
TAACGCA1504.3992717E-528.9988144
GTGGTAT8800.028.0668241
ATAACGC2001.0655956E-525.373963
TGGTATC8900.025.2517762
GAGTACA20650.024.9767131
Produced by FastQC (version 0.11.5)