FastQCFastQC Report
Thu 2 Jun 2016
DRR028322_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028322_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040649
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11800.046.070611
GTATCAA17750.044.919931
ATCAACG17300.044.831413
TCAACGC17200.044.2492184
GACGACC6400.038.50735145
CAACGCA20700.036.7674675
AACGCAG23000.033.7210166
TATCAAC23900.033.0577552
GTACCGA1102.4392904E-432.947476
GTACATG35850.032.754471
ACGCAGA24700.031.1066827
CGCAGAG24650.030.8757218
TACATGG36700.030.02082
TATAACG1000.00608094528.9937732
TATACCG1606.838391E-527.181665
ACCGTAC1100.00968189226.3579771
ACATGGG40750.025.7920063
GAGTACA28650.025.0469761
GTATACG1450.001228013324.994631
GCAGAGT29300.024.9861019
Produced by FastQC (version 0.11.5)