FastQCFastQC Report
Thu 2 Jun 2016
DRR028322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040649
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.12059781924549007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.11723453344979912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.073.078321
GTATCAA26850.054.9201771
TCAACGC29750.049.2252774
ATCAACG30350.048.0132523
CAACGCA31300.046.324365
AACGCAG33450.043.346866
TATCAAC34200.042.8202322
ACGCAGA37350.038.4324767
CGCAGAG37950.037.824858
GTACATG37250.035.881591
TACATGG38500.032.576852
GCAGAGT45600.031.3202469
ACATGGG39750.031.0052743
GAGTACA28000.029.5751511
TATACGG1306.484371E-427.8837012
AGTACAT29750.025.1000182
TATCGTT1500.001493344824.16936145
GTGGTAT7700.023.5846521
CAGAGTA42600.022.8044179
ACCGTAT2003.103843E-421.7492898
Produced by FastQC (version 0.11.5)