FastQCFastQC Report
Thu 2 Jun 2016
DRR028321_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028321_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014255
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16800.051.3421751
TCAACGC15700.051.2459954
ATCAACG16450.048.4689183
GGTATCA11350.047.257681
CAACGCA18750.042.5233965
AACGCAG19950.040.3289226
TATCAAC20850.038.935752
ACGCAGA22850.034.5761647
GTACATG36050.034.3817021
CGCAGAG23350.033.5253568
TACATGG37950.030.5593952
ACATGGG40200.027.7671453
ACACCGG1658.4320716E-526.3574816
GCAGAGT28950.026.0388229
GAGTACA27300.024.9575391
CCTATAC4700.024.6750853
TAATACG2101.5579124E-524.1610224
GACGAAA3402.5120244E-923.450403145
AGAGTAC23900.022.4424555
AGTACAT29100.022.4174422
Produced by FastQC (version 0.11.5)