FastQCFastQC Report
Thu 2 Jun 2016
DRR028321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014255
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.11111604083785635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.11082025723314157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.066.813841
GTATCAA25850.052.8109441
TCAACGC29650.044.7451484
ATCAACG29900.044.371023
CAACGCA31800.041.719925
AACGCAG33150.040.8956956
TATCAAC34800.038.540032
GTACATG36700.035.0214271
ACGCAGA38250.034.495277
TAGATCG1301.6552856E-533.4601175
TACATGG38200.033.0221562
CGCAGAG40050.032.944928
ACATGGG40250.029.7192343
GCAGAGT45850.028.6192849
TATAACG1306.4846245E-427.883432
GAGTACA28500.027.7720721
CATGGGG32750.025.4569344
AGTACAT30650.024.362752
GTACTAG3152.8174327E-823.0523991
CAGAGTA44100.022.8505029
Produced by FastQC (version 0.11.5)