FastQCFastQC Report
Thu 2 Jun 2016
DRR028320_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028320_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009334
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.048.524371
GTACATG39300.040.391451
GTATCAA17500.039.348071
TACATGG39700.037.793552
ATCAACG17900.037.658913
TCAACGC17950.037.1502084
ACATGGG43600.033.9141963
CAACGCA21150.031.529375
AACGCAG22500.029.9597536
TATCAAC22900.029.4364412
CATGGGG33800.028.0926344
ACGCAGA25400.027.6806241
GTGGTAT7350.027.612681
CCGCGCA1050.007717166627.6126779
TGACGCG1606.836622E-527.182575145
CGCAGAG26300.026.4577752
GAGTACA28750.024.959461
AGAGTAC27800.023.205085
AGTACAT30700.021.9574782
CAGAGTA30950.021.7801134
Produced by FastQC (version 0.11.5)