FastQCFastQC Report
Thu 2 Jun 2016
DRR028320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009334
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.15861944609019413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15080.1494054495340492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.077.3531951
GTATCAA30800.053.5551451
ATCAACG34200.047.4827233
TCAACGC34450.046.7172664
CAACGCA35750.045.018465
TATCAAC38400.042.28932
AACGCAG38700.041.7741436
GTACATG39750.036.561071
ACGCAGA44500.036.3294267
CGCAGAG45750.035.1783568
TACATGG42300.033.0773472
GTGGTAT9900.033.0296061
GCAGAGT50850.031.365019
ACATGGG43500.030.9982623
GAGTACA31500.030.2194651
AGTACAT31600.027.0712662
TGGTATC11300.025.662282
CATGGGG32800.024.754714
CATGGGA15400.024.0083964
CTACACT6650.021.8032884
Produced by FastQC (version 0.11.5)