FastQCFastQC Report
Thu 2 Jun 2016
DRR028319_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028319_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861589
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC2550.054.007496145
TCAACGC13550.047.6092534
GGTATCA9400.046.2662241
ATCAACG14250.045.779213
GTATCAA15100.044.1622851
CAACGCA15250.042.301995
AACGCAG15950.039.5365946
GTACATG28050.038.7613641
TACATGG29650.035.9362952
TATCAAC19050.034.6247832
ACGCAGA17800.034.6130267
ACATGGG31750.033.1028183
CGCAGAG18500.032.911548
GAGTACA21850.028.8608061
CATGGGG23550.026.469654
TAGTACG1658.4300875E-526.3577274
AGTACAT21600.026.1746862
TCTCGGG6900.025.211739145
GCAGAGT23700.024.4670899
TAGATCG1500.001496057324.1612515
Produced by FastQC (version 0.11.5)