FastQCFastQC Report
Thu 2 Jun 2016
DRR028319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861589
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14400.069.614031
GTATCAA23150.050.8328171
TCAACGC23300.050.7170564
ATCAACG23600.050.072353
CAACGCA24550.048.1347245
AACGCAG26300.045.2074976
TATAACG1053.824449E-641.427082
TATCAAC29300.040.331312
ACCGTCC1105.2647556E-639.5440338
TACCGTC1105.2647556E-639.5440337
ACGCAGA30450.039.046217
CGCAGAG31100.038.2301338
GTACATG32600.036.5431821
GCAGAGT36700.032.3966569
TACATGG34300.031.915762
ACATGGG35800.030.1734943
GAGTACA24400.029.1753731
GGATACG1000.006016720629.0562861
TACACCG2654.5583874E-927.3575065
TACCTGG9400.026.9937082
Produced by FastQC (version 0.11.5)