FastQCFastQC Report
Thu 2 Jun 2016
DRR028318_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028318_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298540
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.049.3220371
GTATCAA21550.043.728531
TCAACGC21200.043.0827564
ATCAACG21250.042.6402633
GTACATG49950.037.876851
TACATGG52300.034.7889142
TATCAAC28050.033.8537832
CAACGCA27000.033.827945
AACGCAG28750.032.0209856
ACATGGG55400.030.8795573
ACGCAGA30650.029.3264857
GAGTACA37350.029.1118281
CGCAGAG33200.027.0748
AGTACAT39600.024.711972
CATGGGG41300.024.5723554
TAGCGCA1550.001810456623.383379
GCAGAGT39650.021.7556749
ATACCGT2003.1070726E-421.7465346
AGAGTAC34250.020.741225
GTATAGG7100.020.4192811
Produced by FastQC (version 0.11.5)