FastQCFastQC Report
Thu 2 Jun 2016
DRR028318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298540
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.10835245737520599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.10057449135182589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.065.714841
GTATCAA36100.060.180121
ATCAACG40050.053.580563
TCAACGC40150.053.2665444
CAACGCA42700.050.085525
TATCAAC45150.047.688842
AACGCAG45200.047.4756936
ACGCAGA51150.041.1027077
CGCAGAG52750.039.7185558
GTACATG50750.035.506241
GCAGAGT61150.033.788319
TACATGG52850.032.9218522
ACATGGG54300.030.7076133
GTGGTAT12000.030.2745591
GAGTACA38100.027.0802361
TGGTATC13500.025.7765942
CATGGGG41600.024.746464
CCCTATA5050.024.4048212
AGTACAT40550.024.135762
ACCGCGT1851.8316468E-423.5124328
Produced by FastQC (version 0.11.5)