FastQCFastQC Report
Thu 2 Jun 2016
DRR028317_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028317_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990639
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17850.050.7574231
GGTATCA11100.048.9740561
ATCAACG17700.048.3210683
TCAACGC17700.048.3210684
CGATACC4050.044.74173145
CAACGCA19950.042.8713235
AACGCAG21150.040.7816126
TATCAAC22750.038.869252
GTACATG37850.037.9163971
ACGCAGA22950.036.9514057
TACATGG39300.035.779722
GTCGTAC1251.2672766E-534.791171
CGCAGAG25150.033.7190748
ACATGGG42200.032.6338963
CATGGGG32000.027.18064
GCAGAGT29750.027.0435569
GTTCGGG2156.138089E-726.96991
GAGTACA26050.024.7633881
CGTCGTC2651.3796853E-724.616394145
AGTACAT28500.022.6346052
Produced by FastQC (version 0.11.5)