FastQCFastQC Report
Thu 2 Jun 2016
DRR028317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990639
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.1282000809578464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.11901409090496134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.074.02561
GTATCAA27750.057.5796621
ATCAACG29350.052.8597683
TCAACGC29450.052.6802754
CAACGCA30550.050.5461355
AACGCAG31950.048.3312766
TATCAAC32650.047.5171242
ACGCAGA37800.040.659657
GTACATG37000.039.651451
CGCAGAG40200.038.051878
TACATGG38200.037.576942
ACATGGG39350.035.1891023
GCAGAGT45200.033.3614129
CATGGGG31050.031.0534324
TACCTGG11300.029.512022
GAGTACA26200.027.1665331
GTACCTG14100.025.7549271
GTACACG2051.2726063E-524.800111
AGTACAT27050.024.3889832
ACCTGGG13950.023.9057943
Produced by FastQC (version 0.11.5)