FastQCFastQC Report
Thu 2 Jun 2016
DRR028316_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028316_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103746
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12850.045.683444
ATCAACG13950.042.600683
GGTATCA11350.042.1429561
CAACGCA14350.040.908175
AACGCAG16100.037.3619166
TCTCGGG9300.035.067635145
GTATCAA17750.034.7054331
ACGCAGA17600.033.765887
GTACATG30550.033.449131
CGCAGAG18050.032.1210488
TACATGG31900.030.8976292
ACATGGG29850.030.8344543
TATCAAC21250.029.6713472
CATGGGG19900.025.857244
TGTACCG1500.001497602624.1577035
GCAGAGT25150.022.1885879
TAACGCA2353.7202575E-521.5877364
TATAACG2353.7202575E-521.5877362
GTATAAC6400.021.5154551
GAGTACA22500.021.258781
Produced by FastQC (version 0.11.5)