FastQCFastQC Report
Thu 2 Jun 2016
DRR028316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103746
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.057.1736761
TCAACGC20150.046.052664
ATCAACG20850.044.5065353
GTATCAA21850.043.8623661
CAACGCA21450.043.2615935
AACGCAG23150.040.7110376
GTACATG30050.036.7255821
ACGCAGA26300.035.5593457
CGCAGAG26950.034.4326978
ACATGGG28750.033.5377243
TACATGG30350.033.4417652
TATCAAC28000.033.4003872
CGCAGTA1601.8779647E-631.7174222
GAATCGT1358.085224E-426.8507276
GCAGAGT34050.026.8270729
TCCGGTC1658.421745E-526.3625348
CATGGGG19200.024.9208324
CCGGTCT1801.5208565E-424.1656559
AAGGGCG1550.001809047523.386125
GAGTACA20350.023.1909481
Produced by FastQC (version 0.11.5)