FastQCFastQC Report
Thu 2 Jun 2016
DRR028315_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028315_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160058
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15000.047.3584064
ATCAACG15150.046.8895033
GTATCAA17600.043.2452931
CAACGCA18150.039.1391755
GGTATCA11050.038.703651
AACGCAG19450.036.523196
GTACATG35000.032.9299661
ACGCAGA22350.031.1355087
TATCAAC23100.031.0660062
CGCAGAG21750.030.994598
TACATGG35950.029.640172
GAGTACA24300.029.5318831
GAGCGAC3450.029.415157145
ACATGGG36000.029.1962933
TCTCGGG7900.025.69172145
GTGGTAT8650.024.3021161
TATACTG11100.023.509415
TAGATCG1851.8330794E-423.5094095
CATGGGG27100.023.2708474
GCAGAGT28450.022.9309719
Produced by FastQC (version 0.11.5)