FastQCFastQC Report
Thu 2 Jun 2016
DRR028315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160058
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.066.074711
GTATCAA27250.049.6105881
TCAACGC27400.048.4197654
ATCAACG27650.047.981973
CAACGCA28750.046.3983045
AACGCAG30550.043.901836
TATCAAC34750.038.178462
GTACATG36500.037.6354331
ACGCAGA35100.037.591227
CGCAGAG35550.036.911458
TACATGG37150.034.1508372
ACATGGG36100.034.1400263
CATGGGG27500.030.3170724
GCAGAGT42400.030.2642239
TAGATCG1358.085224E-426.850875
GAGTACA25800.025.9176771
TATAACG1701.03168815E-425.5873012
GTGGTAT10550.024.8015021
ATAACGC2252.652764E-522.5547313
TACCTGG11300.021.1717932
Produced by FastQC (version 0.11.5)