FastQCFastQC Report
Thu 2 Jun 2016
DRR028314_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028314_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422392
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC8050.065.73082145
TCAACGC18450.045.9654544
ATCAACG18700.045.7385563
GGTATCA14800.043.0985871
GTATCAA22400.040.7722471
GTACATG42050.039.1292881
CAACGCA22350.037.9446375
AACGCAG24550.034.5443046
TACATGG45350.033.8844682
ACGCAGA25500.032.973117
ACATGGG44750.032.5570533
CGCAGAG25400.032.246818
TATCAAC26750.032.2452132
GAGTACA32600.027.1259081
TACCGTC1358.0948364E-426.845927
CATGGGG32600.024.2354434
ACCGTCC1851.833939E-423.508328
GCAGAGT34300.023.2455949
AGTACAT35100.023.1287922
CTAGACA10250.021.9219884
Produced by FastQC (version 0.11.5)