FastQCFastQC Report
Thu 2 Jun 2016
DRR028314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422392
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.058.0914461
GTATCAA29150.050.5684551
TCAACGC29900.048.4929054
ATCAACG30050.048.49213
CAACGCA31700.045.5106665
AACGCAG33150.043.5200046
TATCAAC37700.039.2290842
ACGCAGA38950.036.853367
CGCAGAG39900.035.430818
GTACATG43650.034.602011
ACATGGG44800.030.5846253
TACATGG46850.030.4842852
CGCTATA1804.7033664E-628.1932352
AACCGCG1050.00771114327.6178637
GCAGAGT51400.027.5036839
GAGTACA33200.026.2461361
CATGGGG31800.026.2174264
AGTACAT36650.023.5392382
GTGGTAT12450.022.746651
TATACCG1600.00217614122.655285
Produced by FastQC (version 0.11.5)