FastQCFastQC Report
Thu 2 Jun 2016
DRR028313_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028313_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556207
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20750.045.760274
ATCAACG20950.045.323423
GTATCAA24350.041.3762631
GGTATCA16900.038.600351
CAACGCA25500.037.5205465
AACGCAG27500.035.58256
GTACATG51450.035.2200551
CGCAGAG28800.033.2213178
ACGCAGA29350.032.845737
ACATGGG48650.032.7774543
TACATGG51950.032.369642
GACGAAA5400.032.21461145
TATCAAC30650.031.9255752
TCTCGGG13700.030.15711145
CATGGGG33750.026.8455094
GCAGAGT36150.026.0657659
GAGTACA37600.024.0967031
ATACCGT3106.2627805E-721.0434156
GACCGGT1750.003664645620.7093937
CTATACA9250.019.5899684
Produced by FastQC (version 0.11.5)