FastQCFastQC Report
Thu 2 Jun 2016
DRR028313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556207
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.060.298571
GTATCAA31150.053.37531
TCAACGC31000.051.449554
ATCAACG31600.050.4726643
CAACGCA31850.050.076485
AACGCAG35700.045.0822266
TATCAAC39100.041.1620332
ACGCAGA40350.039.7072037
CGCAGAG41300.038.6183058
GTACATG51050.036.693311
TACATGG50400.035.8170172
ACATGGG49350.034.0817153
GCAGAGT51200.030.301529
CATGGGG34650.025.5256734
ACACCGC2301.1041611E-625.2163816
ACCGCGT1450.00122703724.9989978
ATACGAC1550.00180936123.386163
GAGTACA37150.022.8659881
GTATATA12650.021.8100191
CATGGGA21550.021.5304574
Produced by FastQC (version 0.11.5)