FastQCFastQC Report
Thu 2 Jun 2016
DRR028312_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028312_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978927
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.049.6697651
GTATCAA16150.046.232661
ATCAACG16100.045.025473
TCAACGC16200.044.747544
CAACGCA19150.038.6113975
GTACATG33700.038.5041281
TGACGCG951.02962666E-438.153164145
AACGCAG20000.036.2455026
TACATGG35450.034.9674532
ACGCAGA21300.032.6720057
ACATGGG37400.031.7875023
TATCAAC22850.031.4074822
CGCAGAG23150.030.0610668
GAGTACA25150.028.2469941
CATGGGG27850.027.8511264
AGTACAT27550.023.94442
GCAGAGT28200.023.906619
GTATTAG8200.020.3328461
GTGGTAT6500.020.0744341
CAGAGTA25750.019.4247769
Produced by FastQC (version 0.11.5)