FastQCFastQC Report
Thu 2 Jun 2016
DRR028312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978927
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.13504582057701953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.134739362587813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.071.775691
GTATCAA28950.061.7168161
TCAACGC32150.054.569764
ATCAACG32750.053.5700073
CAACGCA33450.052.4489635
AACGCAG35100.050.1899576
TATCAAC37200.047.1617662
ACGCAGA39200.044.0157977
CGCAGAG41200.041.8791058
GTACATG37750.039.0567471
GCAGAGT48650.035.4659659
TACATGG40550.035.22032
ACATGGG40050.034.3928953
GTGGTAT9800.031.1272091
TGGTATC10200.027.7192882
GAGTACA26850.027.0503391
GTATACG1357.99834E-426.9000591
CTCGTAC1100.009587293526.4109651
CATGGGA14650.026.227444
CATGGGG30050.025.8140984
Produced by FastQC (version 0.11.5)