FastQCFastQC Report
Thu 2 Jun 2016
DRR028309_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028309_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448945
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC5850.070.62145
ATCAACG22200.049.2983933
TCAACGC22450.048.7494134
GGTATCA14400.047.815631
GTATCAA24650.045.8687021
CAACGCA27850.039.5573465
GTACATG53000.037.6067351
AACGCAG30650.036.18016
ACGCAGA30450.035.9416857
TATCAAC31550.035.8371962
CGCAGAG31000.035.070218
TACATGG56350.033.5703122
ACATGGG58200.032.2541433
TAGCGTA1352.1472251E-532.212649
CGACGAA3950.029.358349145
GAGTACA37850.027.7658461
ACCGATC1606.844066E-527.1794138
GCAGAGT39200.027.179419
CATGGGG41850.026.670684
AGTACAT42300.023.8167882
Produced by FastQC (version 0.11.5)