FastQCFastQC Report
Thu 2 Jun 2016
DRR028309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448945
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.067.857791
GTATCAA33050.055.151451
TCAACGC35350.050.040324
ATCAACG35800.049.6138343
CAACGCA38150.046.367645
AACGCAG40750.043.4092146
TATCAAC44250.040.6310582
ACGCAGA45600.037.8383187
CGCAGAG46850.036.828768
GTACATG53750.036.0734331
TACATGG54250.033.0078282
ACATGGG55850.030.634343
GCAGAGT57700.029.5264859
GTGGTAT10800.028.241011
GTATACG2104.9176015E-727.6646631
GTCTACG3700.027.4777411
GAGTACA40100.025.5346221
TATAACG1450.001227010624.9989472
CATGGGG43050.024.7550544
AGTACAT42700.022.5810152
Produced by FastQC (version 0.11.5)