FastQCFastQC Report
Thu 2 Jun 2016
DRR028308_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028308_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393022
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18900.046.405674
ATCAACG19200.045.680583
GTATCAA21250.043.6614841
GGTATCA14900.042.8098681
GTACATG53500.042.4070471
TACATGG52550.040.2770462
CAACGCA24650.035.580825
ACATGGG58700.034.9458663
TCTAGCG1051.8563613E-434.5166133
TATCAAC26800.033.5377852
ACGCAGA26050.032.277347
AACGCAG26800.032.1854556
GAGTACA37750.032.0661621
CGCAGAG26950.030.6615128
CATGGGG41050.029.6649484
AGTACAT39050.029.142452
CTAGCGT1050.00771731927.613294
GTATAGG8150.024.01341
GCAGAGT33350.023.9080589
CATGGGA19950.023.2532984
Produced by FastQC (version 0.11.5)