FastQCFastQC Report
Thu 2 Jun 2016
DRR028308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393022
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18900.063.4120641
GTATCAA33750.056.6019861
TCAACGC36200.052.0699924
ATCAACG36450.051.712863
CAACGCA38800.048.2070665
AACGCAG41700.044.8545386
TATCAAC44550.043.1241842
GTACATG52800.041.407811
ACGCAGA46350.039.885337
CGCAGAG46600.039.3602078
TACATGG54700.038.1705062
ACATGGG57250.035.3306353
GCAGAGT54500.032.9896669
TAGACCG900.00362934132.2210925
CATGGGA20200.028.7118634
GAGTACA40600.028.4459721
AGTACAT42200.026.628212
CATGGGG40100.026.0339984
ATACCGC1801.5210664E-424.165827
TATACTG8500.023.8815145
Produced by FastQC (version 0.11.5)