FastQCFastQC Report
Thu 2 Jun 2016
DRR028307_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028307_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549618
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19950.051.9565244
ATCAACG20450.050.686193
GTATCAA24200.045.8271681
CAACGCA25700.040.3320125
AACGCAG27300.037.968236
GTACATG55950.036.663451
GGTATCA16250.036.5769461
TACATGG57400.035.358442
TATCAAC30300.035.1658942
ACGCAGA30700.033.763287
ACATGGG59950.031.5571863
CGCAGAG32900.031.5055548
GAGCGAC6100.029.706886145
GAGTACA41700.028.1595631
AGTACAT43750.025.348972
GCAGAGT41650.024.8867389
CATGGGG47950.024.6402994
GTCGTAT1600.002178338122.65151
ATGGGGG29200.019.858855
AGAGTAC34800.019.7875185
Produced by FastQC (version 0.11.5)