FastQCFastQC Report
Thu 2 Jun 2016
DRR028307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549618
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.060.91851
GTATCAA29900.048.584341
ATCAACG32800.042.4376563
TCAACGC33050.042.1166464
CAACGCA34750.040.056265
GTACATG56350.038.9269681
AACGCAG36750.038.0735976
TACATGG56700.037.079932
TATCAAC40150.034.8494342
ACATGGG59250.034.627573
ACGCAGA43100.032.464157
CGCAGAG45350.030.6936058
CATGGGG45400.029.2226264
GAGTACA40500.027.6186981
AGTACAT42850.025.0400332
GCAGAGT55600.025.0351649
ACCGGAC1700.003094131421.3228423
CCTATAC6400.020.3899673
GTGGTAT13950.020.3061661
AGAGTAC48450.020.2005865
Produced by FastQC (version 0.11.5)