FastQCFastQC Report
Thu 2 Jun 2016
DRR028306_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028306_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831767
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12300.050.685154
ATCAACG12450.050.0744863
TATAACG1056.911068E-848.3277052
GTATCAA13500.046.7167781
CAACGCA14350.043.4444165
GTACATG27500.041.6496931
GACGACC4700.041.646587145
AACGCAG15550.040.0917936
GGTATCA9700.039.6087881
TATCAAC16100.038.7221952
TACATGG28600.037.766582
TAACGCA1406.6138637E-736.2457774
ACGCAGA17250.036.1407177
ACATGGG29800.035.0294763
TACCTGG9600.034.7355352
CGCGACC850.002742479334.115723145
CGCAGAG18650.033.427748
ACCGGTC950.004729537330.5227588
GTACGAC950.004729537330.5227583
GTACCTG11300.030.1513561
Produced by FastQC (version 0.11.5)