FastQCFastQC Report
Thu 2 Jun 2016
DRR028306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831767
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.15917919321156046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.15220608656029871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.070.299911
GTATCAA25350.054.4583971
TCAACGC26400.051.3522954
ATCAACG26850.050.491643
CAACGCA28100.047.987575
AACGCAG29850.045.4171036
TATCAAC30550.044.6137582
ACGCAGA33700.040.0133747
GTACATG25850.039.351081
CGCAGAG34750.038.804348
TACATGG27900.035.8589062
ACATGGG29200.033.2693373
GCAGAGT41350.031.9093579
TACCTGG11100.031.3502062
GTGGTAT7450.031.2092191
TAGCGTG950.004727686830.5252029
CATGGGG21600.030.2072324
ACCTGGG11750.029.6159423
GTACCTG12600.028.8329241
GAGTACA19100.028.5310061
Produced by FastQC (version 0.11.5)