FastQCFastQC Report
Thu 2 Jun 2016
DRR028305_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028305_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118668
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15250.051.3284344
ATCAACG15700.050.3188743
CAACGCA17400.044.569595
AACGCAG18200.042.2122576
GGTATCA12050.040.9002531
GTATCAA20450.039.694361
GTACATG37450.036.964571
TATCAAC22100.036.7307552
TACATGG37850.035.0420342
ACGCAGA21750.034.655987
CGCAGAG22350.033.4013338
ACATGGG39100.033.3656733
GAGTACA28350.030.1670631
CATGGGG28350.026.5879214
AGTACAT30850.026.547732
GCAGAGT28050.026.0971229
TCTCGGG10350.025.210745145
GCTAGAC2901.1948941E-824.9922943
GCCGACA3052.0409061E-823.764227145
CATGGGA15350.022.6640224
Produced by FastQC (version 0.11.5)