FastQCFastQC Report
Thu 2 Jun 2016
DRR028305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118668
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.058.6343151
GTATCAA24050.044.9823721
TCAACGC23900.042.7711874
ATCAACG24050.042.2029723
CAACGCA26050.039.2411275
AACGCAG27700.036.903666
GGACCGT1803.2014214E-936.249346
GTACATG38450.034.7450261
TATCAAC30800.033.895492
TACATGG38800.032.3254432
ACGCAGA33150.030.3991457
ACATGGG40300.030.2227763
CGCAGAG33500.030.0815418
GAGTACA28200.029.3512731
TACCTGG11600.026.2495232
CATGGGG29400.025.8923854
GCAGAGT39850.025.2881229
AGTACAT30150.025.2482992
GTATAGG6450.024.764761
GACCGTG2051.2906248E-524.7556467
Produced by FastQC (version 0.11.5)