FastQCFastQC Report
Thu 2 Jun 2016
DRR028304_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028304_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066713
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.049.668041
GTATCAA16500.042.609861
ATCAACG15750.041.8777283
TCAACGC15750.041.8777284
GTACATG38200.038.89671
TACATGG39900.035.4228942
CAACGCA18850.034.9906775
TGACGCG1957.008566E-933.454197145
ACATGGG41050.033.371133
AACGCAG20150.032.733216
TATCAAC20950.032.1751982
ACGCAGA21550.030.2703577
GAGTACA29450.030.0259551
CGCAGAG22800.028.29298
CATGGGG32750.027.6643854
AGTACAT30000.026.0930462
GTGGTAT7450.024.322381
GTATAGG4205.2750693E-1122.43451
GTATATA7550.022.080211
GCAGAGT28600.021.5414629
Produced by FastQC (version 0.11.5)