FastQCFastQC Report
Thu 2 Jun 2016
DRR028304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066713
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.11268260534933014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.1069641037467435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.067.152991
GTATCAA27000.058.9110031
ATCAACG29200.053.379813
TCAACGC29550.052.7475664
CAACGCA31400.049.6398285
AACGCAG32550.047.886046
TATCAAC34000.046.0570682
ACGCAGA37500.041.3717547
CGCAGAG38650.039.5780538
GTACATG37250.038.0210761
TCTAGCG1251.2654977E-534.798673
TACATGG39600.034.4178772
GCAGAGT46200.033.2671289
ACATGGG40900.032.9694023
GAGTACA28350.031.7676221
AGTACAT28550.028.1863162
GTGGTAT9200.026.0520691
CATGGGG32300.026.036164
GAACGGT1450.0012267724.9990466
CAGAGTA42300.021.4235329
Produced by FastQC (version 0.11.5)