FastQCFastQC Report
Thu 2 Jun 2016
DRR028303_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028303_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814057
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8400.050.90951
ATCAACG11750.044.4140933
TCAACGC12050.043.909854
CGACGAC2200.042.829872145
GTATCAA13550.040.6537321
GTACATG25150.037.1773031
CAACGCA14100.037.011745
TATCAAC15550.035.8910752
AACGCAG15300.035.0563286
TACATGG27350.033.6567762
TACCTGG8850.031.9409242
ACGCAGA17150.031.2747447
CGCAGAG17100.030.9423248
ACATGGG29450.029.287873
GGACCGT2003.2604294E-728.9925336
GAGTACA20150.028.0572871
ACCGATC1050.007717317427.6119338
AGTACAT20650.026.6759382
CATGGGG21200.025.9838714
GTACTCG1400.001000802125.8861891
Produced by FastQC (version 0.11.5)