FastQCFastQC Report
Thu 2 Jun 2016
DRR028303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814057
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.056.738141
GTATCAA20700.054.4069181
ATCAACG21700.049.7803463
TCAACGC21750.049.665914
CAACGCA22950.047.0695
TATCAAC24750.044.5245742
AACGCAG25200.043.44186
ACGCAGA28850.037.9456947
CGCAGAG28900.037.3783238
GTACATG27150.037.199551
TACATGG27850.034.882772
ACATGGG28550.033.7735633
TACCTGG9300.033.5209962
GCAGAGT33550.031.7655359
GTTAACG1601.8767732E-631.718266145
CATGGGG21300.029.6122264
ACCTGGG10900.029.265613
GTACCTG11650.027.4422361
GAGTACA19250.026.0442051
ATGGGGG15600.023.7015615
Produced by FastQC (version 0.11.5)