FastQCFastQC Report
Thu 2 Jun 2016
DRR028302_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028302_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277507
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.049.5170061
GTATCAA20150.049.2831081
ATCAACG18900.048.3238223
TCAACGC18850.048.0674634
GCGTTAG657.344466E-444.6066061
CAACGCA23500.038.556245
TATCAAC24350.038.1033822
GTACATG46650.037.7578431
TACATGG47850.036.3564832
AACGCAG26050.035.0602766
ACGCAGA26950.033.8894357
CGCAGAG26850.033.745698
ACATGGG50200.032.7774123
GAGTACA36100.028.7131841
CATGGGG37200.026.889874
AGTACAT36650.025.7111722
GCAGAGT33700.025.3807629
TAGATCG1450.00122803824.9950795
CCGCGCG2303.147682E-522.0608759
GTATTAG11850.022.0209831
Produced by FastQC (version 0.11.5)