FastQCFastQC Report
Thu 2 Jun 2016
DRR028302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277507
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.062.648921
GTATCAA28150.054.915171
ATCAACG30700.050.5347753
TCAACGC31300.049.5660554
CAACGCA33500.046.310975
AACGCAG34950.044.804486
TATCAAC36800.041.9610822
GTACATG41700.039.1595421
ACGCAGA40150.038.4599727
TACATGG43300.036.4992142
CGCAGAG42300.036.3337638
ACATGGG45900.033.3261153
TTTCGCG1652.388104E-630.7559389
GCAGAGT50950.029.596079
TATACCG1255.1562354E-428.9984595
CATGGGG33850.028.0561164
TAGATCG1358.0864574E-426.8504245
CGTATGG1100.00967507926.3622342
GTCGTAC1450.001219191225.0260981
CAGAGTA47350.024.19099
Produced by FastQC (version 0.11.5)