FastQCFastQC Report
Thu 2 Jun 2016
DRR028301_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028301_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932750
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC4500.069.26376145
GTATCAA16100.053.576121
TCAACGC15450.051.6076854
ATCAACG15600.051.5761033
GGTATCA10150.047.847471
CAACGCA18300.043.5704235
TATCAAC20200.040.907572
AACGCAG19400.040.72636
GTACATG35000.039.9704861
ACGCAGA20550.037.389037
CGCAGAG21000.037.2781758
TACATGG37300.036.5341642
ACATGGG38050.034.480543
CGACGAA2950.031.942692145
TAGAACG1204.0626485E-430.2022274
GCAGAGT26600.028.6126359
CGATGCG1050.007715827.613464145
GAGTACA26300.027.2853571
CATGGGG30050.027.2573834
AGTACAT27000.024.4302442
Produced by FastQC (version 0.11.5)