FastQCFastQC Report
Thu 2 Jun 2016
DRR028301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932750
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.059.2851871
GTATCAA19650.051.7328531
TCAACGC22550.044.0452774
ATCAACG22500.043.8209423
CAACGCA23850.041.6444855
AACGCAG25400.039.3886156
TATCAAC27300.036.116162
GTACATG36050.035.247941
ACGCAGA29500.033.6685077
TACATGG36650.033.232322
TAGTGCG900.003628625532.2212839
CGCAGAG31100.031.7032538
CTACGCT950.00472780930.5254254
GAGTACA26200.030.4854341
ACATGGG39250.029.9226913
CGTATAC1255.1544595E-428.9991533
GTGGTAT9150.028.5681671
AGTACAT27850.027.8537652
GCAGAGT36050.026.5456899
CCTAGAC4650.024.9455093
Produced by FastQC (version 0.11.5)